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Nov 30 Read Oct 12 Read Dec 23 Read Dec 12 Read Dec 20 Read Dec 04 Read Aug 31 Read Aug 25 Read Aug 07 Read Jul chrlmacademy Read Nov 05 Read Feb 28 Read Dec 29 Read chromacadeym Dec 26 Read In chromatography, a response factor is defined as the ratio between the chro,academy of a compound being analysed and the response of the detector to that compound.
A chromatogram will show a response from a detector as a peak. While there are several ways to quantify the chromaacademy, one of the most common is peak area, thus: It is important to remember that variations in a chromxcademy chromatography GC system and analysis methodology can be the cause of a deviation in the response factor.
Response factors are important when using GC for quantitative analysis. Therefore, for reproducibility in sample measurements, a method of eliminating variability in the response factor must be used. One of the easiest ways to eliminate variation in the response factor is to use relative response factors and an internal standard to calibrate the GC. The response factors calculated for each analyte are then used to establish the RRF between the two analytes thus: The RRF can be used to calculate the unknown concentration of analyte A in the presence of a known concentration of analyte B using the following equation:.
Using RRFs is beneficial when one of the samples chromacavemy an internal standard – a calibration then allows multiple analyses to be run on different samples. An internal standard IS is a compound that is similar in physical and chromaccademy characteristics to the sample being analysed. It must be inert to the sample and must not react with the sample or any solvent used to dilute or prepare it for GC. To determine the ratio of two compounds A and B in a sample, first the GC has to be calibrated.
Once the calibration is complete, the sample can be analysed and the concentrations of A and B in the unknown sample determined. A simplified procedure is:.
Add a known amount of IS. The same concentration of IS should be used in chromaacademy calibration and analysis samples. After analysis, the results required are the peak areas of A, B and IS.
Since we know the peak areas of A and IS from step 3and the concentration of IS used, we can calculate the concentration of A. A similar calculation yields the concentration of B. Chromatography Today Do you like or dislike what you have read?
What is a Response Factor?
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A simplified procedure is: Read comments 0 Do you like or dislike what you have read? Adsorption, Absorption and Desorption – What’s Understanding chrokacademy Difference Between Retention What is Retention Time? Revisiting Data Integrity and Chromatography Da China Lab Feb 26 Guangzhou, China. Israchem Mar 12 Tel Aviv, Israel.